MorphantTM
zebrafish embryos have been injected with morpholino antisense oligonucleotides
to temporarily inhibit expression of a target gene (Ekker, et al.; US
Patent 6,867,349, 2008). Morpholinos (manufactured exclusively by Gene Tools,
LLC) are widely recognized as the standard gene knockdown tool in zebrafish
embryos. A morpholino specifically binds to and functionally inhibits its
selected target by one of two mechanisms: 1) blocking mRNA translation,
or 2) blocking proper pre-mRNA splicing. The ability to obtain gene knockdown
data in a whole organism in less than one week makes the zebrafish model
highly attractive for target validation. Zygogen offers
Morphant Gene Knockdown Embryos injected with morpholinos targeting zebrafish homologs of customer-specified
genes. Morphant Gene Knockdown Embryos are available from wild-type and Z-TagSM
transgenic lines.
Compound and gene effects on the development of specific organs can be easily visualized and assessed using bright-field and fluorescence stereomicroscopy. (Basic Morphological Phenotypes)
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• Cardiovascular System |
• Skeletal System • Ocular System • Embryonic Development • Motility • Morphology of Fins • Pigmentation |