MorphantTM zebrafish embryos have been injected with morpholino antisense oligonucleotides to temporarily inhibit expression of a target gene. Morpholinos (manufactured exclusively by Gene Tools, LLC) are widely recognized as the standard gene knockdown tool in zebrafish embryos. A morpholino specifically binds to and functionally inhibits its selected target by one of two mechanisms: 1) blocking mRNA translation, or 2) blocking proper pre-mRNA splicing. The ability to obtain gene knockdown data in a whole organism in less than one week makes the zebrafish model highly attractive for target validation. Zygogen offers Morphant Gene Knockdown Embryos injected with morpholinos targeting zebrafish homologs of customer-specified genes.
Morphant Gene Knockdown Embryos are available from wild-type and Z-TagSM transgenic lines.
Compound and gene effects on the development of specific organs can be easily visualized and assessed using bright-field and fluorescence stereomicroscopy.
• Cardiovascular System |
• Skeletal System • Ocular System • Embryonic Development • Motility • Morphology of Fins • Pigmentation |