Zygogen has developed a high throughput, quantitative acute toxicity assay using zebrafish embryos. Embryo length provides a reliable surrogate for acute systemic toxicity and correlates well with published rodent LD50 values. Z-Tag fluorescent embryos facilitate automated analysis and allow for rapid, cost-effective compound assessment. This assay can be used to determine the safety of novel therapeutic compounds early in the drug discovery process and to assess the hazard potential and environmental risk of chemicals.
For compound screening in the Z-Tag Acute Toxicity Assay, embryos are treated at 48-52 hours post fertilization, and embryo length is measured 24 hours after treatment.
Download the Z-Tag Safety & Toxicity Brochure (PDF)
Changes in the length and shape of zebrafish embryos serve as a surrogate readout for the overall level of toxicity.
Dose-response curves of seven compounds evaluated in the Z-Tag Acute Toxicity Assay. Abnormality EC50 values are shown for compounds causing toxicity in the dose range tested. Rat acute oral LD50 values are shown for comparison.
Related Links
Z-Tag Acute Toxicity Assay Starter Kit
Z-Tag Acute Toxicity Assay Services
Z-Tag Blood Vessel Embryos